Detection of nanoseismic events related to slope instabilities in the quarry district of Coreno Ausonio (Italy)

Le cave per l’estrazione di materiale roccioso rappresentano contesti in cui possono aver luogo eventi di instabilità gravitativa causati dalle continue sollecitazioni cui sono soggette le pareti produttive, principalmente connesse alle vibrazioni dovute alle esplosioni necessarie alle operazioni di disgaggio. La necessità di gestione del rischio da frana per la salvaguardia del personale impegnato nell’attività estrattiva ha portato, nel tempo, alla richiesta di attivare sistemi di monitoraggio nelle aree di coltivazione mineraria e di cava. Nel presente lavoro il monitoraggio nanosismometrico, una tecnica di geofisica passiva recentemente sviluppata per le indagini di microsismicità, è stato impiegato nel distretto di cave a cielo aperto di Coreno Ausonio (in provincia di Frosinone). Il monitoraggio nanosismometrico consente l’individuazione e la localizzazione di deboli eventi sismici, fino a magnitudo locale (ML) nell’ordine di -3, attraverso l’impiego di quattro sensori sismometrici disposti secondo una specifica geometria di array detta SNS (Seismic Navigation System). Dopo aver individuato una cava in cui erano programmate esplosioni per la volata delle pareti in roccia, nel corso del 2013 sono state organizzate 3 campagne di acquisizione durante tre giornate, pianificate in modo da monitorare l’area in un periodo compreso da qualche ora prima dell’esplosione alle 24 ore successive. Su una parete della cava non più produttiva è stato effettuato un rilevamento geologico-tecnico che ha permesso di individuare 4 principali sistemi di discontinuità e caratterizzarli in termini di giacitura, resistenza, rugosità, apertura, spaziatura e condizioni idrauliche secondo gli standard ISRM (1978). L’analisi di stabilità della parete in esame, tenuto conto della sua orientazione, ha restituito una scarsa propensione ad eventi di instabilità. Analizzando mediante il software NanoseismicSuite i dati sismometrici acquisiti è stato possibile ottenere i “supersonogrammi”, ovvero particolari spettrogrammi auto-adattanti alle variazioni del rumore sismico di fondo, dai quali sono state definite alcune caratteristiche specifiche di forma d’onda per diverse tipologie di eventi. In base ai supersonogrammi, è stato possibile individuare e localizzare 15 esplosioni, di cui 3 provenienti dalla cava di riferimento e 12 da cave adiacenti del distretto, e 27 deboli eventi di instabilità gravitativa, distinti in 23 eventi di collasso e 4 rotture legate alle fratturazione dell’ammasso roccioso. Le 3 esplosioni avvenute nella cava di riferimento, e quindi aventi coordinate di origine nota, sono state utilizzate per calibrare il modello di sottosuolo, successivamente impiegato per localizzare gli altri eventi registrati. Le rotture sono risultate originate in diverse zone del distretto estrattivo, mentre gli eventi di collasso sono stati localizzati in una specifica area e risultano essere avvenuti prevalentemente in un limitato intervallo di tempo a seguito delle 9 esplosioni registrate nella campagna del 26-27 luglio 2013. Non è stato possibile, invece, individuare eventi riconducibili ad instabilità negli orari di attività di cava a causa dell’elevato livello di rumore apportato dagli strumenti per l’estrazione e la lavorazione del materiale roccioso. Si è escluso che gli eventi di collasso fossero riconducibili direttamente all’attività di estrazione sia perché registrati al di fuori dell’orario di lavorazione delle cave sia perché, analizzando l’intera registrazione per intervalli orari, le frequenze tipiche dei macchinari di lavorazione non sono risultate energizzante. La zona di origine è risultata essere un’area nella quale sono stati rinvenuti detrito sciolto costituito da blocchi eterometrici ed una parete non in coltivazione con medesime caratteristiche delle discontinuità rispetto alla parete sulla quale era stato effettuato il rilevamento geologico-tecnico, ma con diversa orientazione. In definitiva, la fase di sperimentazione ha restituito dei risultati di indubbio interesse consentendo di mettere in evidenza alcune limitazioni del monitoraggio nanosismometrico nel contesto preso in esame, in particolare legate all’eccessiva rumorosità registrata nelle ore di attività di cava. La tecnica appare, comunque, un utile strumento di monitoraggio per i fenomeni gravitativi di debole intensità, in grado di contribuire alla gestione del rischio da frana in aree ad elevata attività antropica ed in ambienti naturalmente predisposti ad instabilità gravitative che possono interessare pareti in roccia.Nanoseismic monitoring is a passive geophysical technique used to identify and locate weak seismic events (down to local magnitudes, ML, around -3). This technique was applied in the open-pit quarry district of Coreno Ausonio (central Italy) to detect possible gravity-induced slope instabilities resulting from quarry rock blasting. After identifying an active quarry, an engineering-geological survey was carried out to characterise the jointed rock mass on an abandoned wall in front of the quarry. Four main joint sets were surveyed and their geometric and mechanical properties were measured in order to carry out stability analyses that evidenced scarce proneness to failure of the investigated wall. The analysis of seismic records obtained during three monitoring surveys, performed through the NanoseismicSuite software, made it possible to detect and characterise 15 blasts, of which 3 from the reference quarry and 12 from nearby quarries within the district, as well as 27 weak slope instability events (23 collapses and 4 failures). While failures originated from different areas of the quarry district, collapses occurred in a site characterised by an abandoned quarry having a wall more prone to gravity-induced instabilities than the one previously characterised

Rock mass characterization coupled with seismic noise measurements to analyze the unstable cliff slope of the Selmun Promontory (Malta)

In the Mediterranean area, cliff slopes represent widespread high-risk landforms as they are highly frequented touristic places often interested by landslide processes. Malta represents a significant case study as several cliffs located all around the island are involved in instability processes, as evidenced by wide block-size talus distributed all along the coast line. These diffused instabilities are related to the predisponding geological setting of Malta Island, i.e. the over-position of grained limestone on plastic clay deposits, that induces lateral spreading phenomena associated to falls and topples of different-size rock blocks and is responsible for a typical landscape with stable plateau of stiff rocks bordered by unstable cliff slopes. The ruins of Gƫajn ƪadid Tower, the first of the thirteen watchtowers built in 1658 by the Gran Master Martin de Redin, stand out in the Selmun area. Currently the safety of this important heritage site, already damaged by an earthquake on October 12th 1856, is threaten by a progressive moving of the landslide process towards the stable plateau area. During autumn 2015, a field campaign was realized to characterize the jointed rock mass. A detailed engineering-geological survey was carried out to reconstruct the geological setting and to define the mechanical properties of the rock mass. Based on the surveyed joint spatial distribution, 58 single-station noise measurements were deployed to cover both the unstable zone and the stable area. The obtained 1-hour records were analyzed in the frequency domain for associating vibrational evidences to different instability levels, i.e. deriving the presence of already isolated blocks by the local seismic response. The here presented results can be a useful contribute to begin to asses defense strategies for the Selmun Promontory, in the frame of managing the landslide risk in the study area and preserving the local historical heritage

Investigating rock mass failure precursors using a multi-sensor monitoring system. Preliminary results from a test-site (Acuto, Italy)

In the last few years, several approaches and methods have been proposed to improve early warning systems for managing risks due to rapid slope failures where important infrastructures are the main exposed elements. To this aim, a multi-sensor monitoring system has been installed in an abandoned quarry at Acuto (central Italy) to realise a natural-scale test site for detecting rock-falls from a cliff slope. The installed multi-sensor monitoring system consists of: i) two weather stations; ii) optical cam (Smart Camera) connected to an Artificial Intelligence (AI) system; iii) stress- strain geotechnical system; iv) seismic monitoring device and nano-seismic array for detecting microseismic events on the cliff slope. The main objective of the experiment at this test site is to investigate precursors of rock mass failures by coupling remote and local sensors. The integrated monitoring system is devoted to record strain rates of rock mass joints, capturing their variations as an effect of forcing actions, which are the temperature, the rainfalls and the wind velocity and direction. The preliminary tests demonstrate that the data analysis methods allowed the identification of external destabilizing actions responsible for strain effects on rock joints. More in particular, it was observed that the temperature variations play a significant role for detectable strains of rock mass joints. The preliminary results obtained so far encourage further experiments

Geological constraints for a conceptual evolutionary model of the slope deformations affecting Mt. Nuovo at Ischia (Italy)

ischia island was the scenario of several Holocene slope in- stability events occurred at different scales, from shallow mass movements, triggered by meteo-climatic forcing, up to massive rock slope failures such as large debris avalanches these last ones related to the volcano-tectonic dynamics of a resurgent caldera. the present study focuses on the gravitational deformation that in- volves Mt. nuovo, located in the western portion of Mt. epomeo resurgent block. a high-resolution engineering-geological model was reconstructed according to a multi-modelling approach sup- ported by field geo-structural evidences and constrained by pas- sive seismic investigations. it revealed a complex morpho-struc- tural setting and led to the identification of a multiple compound mechanism, involving a rock mass volume of about 190 million of cubic meters. the obtained geological model shows a partial structural control of the pre-existing tectonic pattern on slope deformation mechanisms, highlighting geometric and volumetric similarities between the Mt. nuovo ongoing deformation and an already oc- curred rock avalanche. the defined conceptual evolutionary mod- el allows to hypothesize the role of inner pressures constraining the shear zone initiation and propagation and making reliable a future scenario of generalized collapse. Starting from these new field and laboratory data, numerical models will be reconstructed in order to depict the evolution of the gravitational slope deformation, evaluate its sensitivity and constrain future evolutionary instability scenarios

Seismic response of the geologically complex alluvial valley at the "Europarco Business Park" (Rome - Italy) through instrumental records and numerical modelling

The analysis of the local seismic response in the “Europarco Business Park”, a recently urbanized district of Rome (Italy) developed over the alluvial valley of the “Fosso di Vallerano” stream, is here presented. A high-resolution geological model, reconstructed over 250 borehole log-stratigraphies, shows a complex and heterogeneous setting of both the local Plio- Pleistocene substratum and the Holocene alluvia. The local seismo-stratigraphy is derived by a calibration process performed through 1D numerical modelling, accounting for: i) 55 noise measurements, ii) 10 weak motion records obtained through a temporary velocimetric array during the August 2009 L’Aquila- Gran Sasso seismic sequence and iii) one cross-hole test available from technical report. Based on the reconstructed seismo- stratigraphy, the local seismic bedrock is placed at the top of a gravel layer that is part of the Pleistocene deposits and it does not correspond to the local geological bedrock represented by Plio-Pleistocene marine deposits. 1D amplification functions were derived via numerical modelling along three representative sections that show how in the Fosso di Vallerano area two valleys converge into a single one moving from SE toward NW. The obtained results reveal a main resonance at low frequency (about 0.8 Hz) and several higher resonance modes, related to the local geological setting. Nonlinear effects are also modelled by using strong motion inputs from the official regional dataset and pointed out a general down-shift (up to 0.5 Hz) of the principal modes of resonance as well as an amplitude reduction of the amplification function at frequencies higher than 7 Hz

Hippocampal Estrogen Signaling Mediates Sex Differences in Retroactive Interference

Despite being a crucial physiological function of the brain, the mechanisms underlying forgetting are still poorly understood. Estrogens play a critical role in different brain functions, including memory. However, the effects of sex hormones on forgetting vulnerability-mediated by retroactive interference (RI), a phenomenon in which newly acquired information interferes with the retrieval of already stored information, are still poorly understood. The aim of our study was to characterize the sex differences in interference-mediated forgetting and identify the underlying molecular mechanisms. We found that adult male C57bl/6 mice showed a higher susceptibility to RI-dependent memory loss than females. The preference index (PI) in the NOR paradigm was 52.7 +/- 5.9% in males and 62.3 +/- 13.0% in females. The resistance to RI in female mice was mediated by estrogen signaling involving estrogen receptor alpha activation in the dorsal hippocampus. Accordingly, following RI, females showed higher phosphorylation levels (+30%) of extracellular signal-regulated kinasel/2 (ERK1/2) in the hippocampus. Pharmacological inhibition of ERK1/2 made female mice prone to RI. The PI was 70.6 +/- 11.0% in vehicle-injected mice and 47.4 +/- 10.8% following PD98059 administration. Collectively, our data suggest that hippocampal estrogen alpha receptor-ERK1/2 signaling is critically involved in a pattern separation mechanism that inhibits object-related RI in female mice

Influence of geological complexities on local seismic response in the municipality of Forio (Ischia Island, Italy)

Seismic response studies carried out in the Municipality of Forio on Ischia (NA), southern Italy, following the 21st August 2017 earthquake allowed to detect local effects related to specific geological-structural settings that reflect the complexity of the volcanic context which characterises the entire island of Ischia and, more specifically, the western sector of Mt. Epomeo and the adjacent coastal plain of Forio. In particular, the following features have been observed: i) polarization and amplification effects in the proximity of tectonic elements that dissect the Zaro promontory, where volcanic deposits from massive to stratified widely outcrop; ii) stratigraphic resonances on significantly variable frequency values, changing within distances of a few hundred meters, which can be related to the juxtaposition of landslide debris (such as debris-/rockavalanches and lahar) in correspondence with the town of Forio; iii) seismic amplification in the sector involved in the ongoing gravitational deformation of Mt. Nuovo even in the absence of polarization of the particle motion. The peculiarities of the geological contexts analysed in the Forio Municipality allow to apply different interpretative schemes that vary from stratigraphic resonance (one-dimensional model of resonant column) mostly controlled by thickness and wave velocity in soft soils onto a stiff bedrock, to the resonance of jointed rock masses which are not completely released from the adjacent bedrock so avoiding typical free vibrations with normal modes (three-dimensional oscillating mass model), or to the interaction in the near surface with physical discontinuities responsible for modifying the physical properties of surface waves polarizing and amplifying them. The collected evidence of local seismic response in Forio exemplifies how not conventional interpretative keys for seismic zoning can be proposed to identify sectors whose response schemes do not necessarily fall within the standard of stratigraphic or topographical amplification adopted by current national guidelines for Seismic Microzonation (SM) studies. In particular, the evidence of local seismic response collected for the Forio Municipality were taken into account in the SM and relative products that were realised in the following year

Transcranial Direct Current Stimulation Enhances Neuroplasticity and Accelerates Motor Recovery in a Stroke Mouse Model

BACKGROUND: More effective strategies are needed to promote poststroke functional recovery. Here, we evaluated the impact of bihemispheric transcranial direct current stimulation (tDCS) on forelimb motor function recovery and the underlying mechanisms in mice subjected to focal ischemia of the motor cortex. METHODS: Photothrombotic stroke was induced in the forelimb brain motor area, and tDCS was applied once per day for 3 consecutive days, starting 72 hours after stroke. Grid-walking, single pellet reaching, and grip strength tests were conducted to assess motor function. Local field potentials were recorded to evaluate brain connectivity. Western immunoblotting, ELISA, quantitative real-time polymerase chain reaction, and Golgi-Cox staining were used to uncover tDCS-mediated stroke recovery mechanisms. RESULTS: Among our results, tDCS increased the rate of motor recovery, anticipating it at the early subacute stage. In this window, tDCS enhanced BDNF (brain-derived neurotrophic factor) expression and dendritic spine density in the peri-infarct motor cortex, along with increasing functional connectivity between motor and somatosensory cortices. Treatment with the BDNF TrkB (tropomyosin-related tyrosine kinase B) receptor inhibitor, ANA-12, prevented tDCS effects on motor recovery and connectivity as well as the increase of spine density, pERK (phosphorylated extracellular signal-regulated kinase), pCaMKII (phosphorylated calcium/calmodulindependent protein kinase II), pMEF (phosphorylated myocyte-enhancer factor), and PSD (postsynaptic density)-95. The tDCSpromoted rescue was paralleled by enhanced plasma BDNF level, suggesting its potential role as circulating prognostic biomarker. CONCLUSIONS: The rate of motor recovery is accelerated by tDCS applied in the subacute phase of stroke. Anticipation of motor recovery via vicariate pathways or neural reserve recruitment would potentially enhance the efficacy of standard treatments, such as physical therapy, which is often delayed to a later stage when plastic responses are progressively lower

Temporary dense seismic network during the 2016 Central Italy seismic emergency for microzonation studies

In August 2016, a magnitude 6.0 earthquake struck Central Italy, starting a devastating seismic sequence, aggravated by other two events of magnitude 5.9 and 6.5, respectively. After the first mainshock, four Italian institutions installed a dense temporary network of 50 seismic stations in an area of 260 km2. The network was registered in the International Federation of Digital Seismograph Networks with the code 3A and quoted with a Digital Object Identifier ( https://doi.org/10.13127/SD/ku7Xm12Yy9 ). Raw data were converted into the standard binary miniSEED format, and organized in a structured archive. Then, data quality and completeness were checked, and all the relevant information was used for creating the metadata volumes. Finally, the 99 Gb of continuous seismic data and metadata were uploaded into the INGV node of the European Integrated Data Archive repository. Their use was regulated by a Memorandum of Understanding between the institutions. After an embargo period, the data are now available for many different seismological studies.Publishedid 1825T. Sismologia, geofisica e geologia per l'ingegneria sismicaJCR Journa

Novel and Rare Fusion Transcripts Involving Transcription Factors and Tumor Suppressor Genes in Acute Myeloid Leukemia.

Approximately 18% of acute myeloid leukemia (AML) cases express a fusion transcript. However, few fusions are recurrent across AML and the identification of these rare chimeras is of interest to characterize AML patients. Here, we studied the transcriptome of 8 adult AML patients with poorly described chromosomal translocation(s), with the aim of identifying novel and rare fusion transcripts. We integrated RNA-sequencing data with multiple approaches including computational analysis, Sanger sequencing, fluorescence in situ hybridization and in vitro studies to assess the oncogenic potential of the ZEB2-BCL11B chimera. We detected 7 different fusions with partner genes involving transcription factors (OAZ-MAFK, ZEB2-BCL11B), tumor suppressors (SAV1-GYPB, PUF60-TYW1, CNOT2-WT1) and rearrangements associated with the loss of NF1 (CPD-PXT1, UTP6-CRLF3). Notably, ZEB2-BCL11B rearrangements co-occurred with FLT3 mutations and were associated with a poorly differentiated or mixed phenotype leukemia. Although the fusion alone did not transform murine c-Kit+ bone marrow cells, 45.4% of 14q32 non-rearranged AML cases were also BCL11B-positive, suggesting a more general and complex mechanism of leukemogenesis associated with BCL11B expression. Overall, by combining different approaches, we described rare fusion events contributing to the complexity of AML and we linked the expression of some chimeras to genomic alterations hitting known genes in AML